Transcription in eukaryotic nuclei is carried out by DNA-dependent RNA polymerases I, II, and III. Human RNA polymerase III (Pol III) transcribes small untranslated RNAs that include tRNAs, 5S RNA, U6 ...
For all species, the process of DNA replication requires the use of multiple different DNA polymerase enzymes. The primary function of these unique multisubunit enzymes is to ensure optimal DNA ...
A rapid and simple, large-scale method for the purification of DNA-dependent RNA polymerase III (EC 2.7.7.6) from wheat germ is presented. The method involves enzyme extraction at low ionic strength, ...
The latest short science news items from C&EN. Ting F. Zhu, who led the work, says it is a step on the way to creating a mirror version of the so-called central dogma of molecular biology, which ...
As expected, accuracy also depended on whether the DNA polymerase retains a 'proofreading' functionality, which verifies (and if necessary removes) any mismatched nucleotides: a version of the enzyme ...
Biology lectures teach students that when a cell’s replication machinery comes together, DNA polymerase takes off down the double-helix like a car on a highway ...
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